Cel AMPA-type glutamate receptors (AMPARs) mediate most fast excitatory synaptic transmission in the brain. The regulation of certain AMPARs (calcium-permeable; CP-AMPARs) is relevant in synaptic plasticity, neuronal development and certain neurological diseases. AMPARs are regulated by transmembrane AMPA regulatory proteins (γ-2, -3, -4, -8; TARPs), which affect the trafficking and also important biophysical properties of AMPARs. Among them, we have recently demonstrated that TARPs increase single-channel conductance and attenuate block by intracellular polyamines of CP-AMPARs (Soto et al., 2007). However, the mechanisms involved in those changes are yet to be determined. On the other hand, new AMPARs auxiliary subunits have been recently discovered, such as γ-5, γ-7 or CNIHs raising interesting questions regarding the role of these proteins in AMPARs function. For example, how do TARPs modify the block of AMPARs by polyamines? How do the new auxiliary subunits affect some biophysical properties of AMPARs? Do TARPs or CNIHs modulate AMPARs in periferic nervous system (motorneurons or Schwann cells)? Therefore, the aim of the proposed research project is to further our understanding of the roles played by AMPARs auxiliary subunits (TARPs and CNIHs). We will address the following issues: 1) Using patch-clamp techniques we will investigate AMPARs modulation by auxiliary subunits and the molecular mechanisms involved in that modulation. 2) With the patch-clamp technique in either expression systems or neuronal cultures, we will study AMPARs auxiliary subunits focusing on the new recently discovered ones. We will take advantage of pharmacological and molecular tools (site-directed mutagenesis and siRNA) combined with the patch-clamp records. 3) The role of auxiliary subunits will be investigated in motor neurons and Schwann cells. We anticipate that the results will shed new light on the AMPAR field that will be invaluable in future improvements for certain CNS conditions. Dziedzina nauki natural sciencesbiological sciencesneurobiologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteins Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2011-CIG - Marie-Curie Action: "Career Integration Grants" Zaproszenie do składania wniosków FP7-PEOPLE-2011-CIG Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-CIG - Support for training and career development of researcher (CIG) Koordynator FUNDACIO INSTITUT D'INVESTIGACIO BIOMEDICA DE BELLVITGE Wkład UE € 100 000,00 Adres AVENIDA GRAN VIA HOSPITALET 199-203 08908 L'Hospitalet De Llobregat Hiszpania Zobacz na mapie Region Este Cataluña Barcelona Rodzaj działalności Research Organisations Kontakt administracyjny Laia Lagunas (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
