Objective
Virus maturation is virtually universal and allows the transition from an initially assembled non-infectious and fragile particle to a robust infectious virion. Siphophage HK97 is an accessible model system, to study assembly and maturation, for which intermediates can be isolated and myriad structural, biophysical and biochemical data are available. The capsid protein fold similarity between HK97, all other dsDNA bacteriophages and some animal viruses suggests that they share common maturation principles. The proposed project seeks to unravel the mechano-chemical reorganization program underlying the structural transitions observed during HK97 procapsid maturation using hybrid methods. It relies on a multi-disciplinary approach involving X-ray crystallography, electron microscopy and tomography to obtain multi-scale data ranging from atom to whole-cell. We aim to obtain structures of three different HK97 procapsid maturation intermediates to shed light on mechanisms involved in initial assembly of an energy-loaded particle as well as on forces driving forward maturation. We will also use electron tomography to study in vivo aspects of HK97 assembly and maturation in Escherichia coli infected cells. The outcome will allow apprehending mechanisms, dynamics and energetics of viral maturation as well as provide a basis for the use of HK97 as a delivery system for nano-medical applications.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences microbiology virology
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-IOF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75724 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.