Objective
Just prior to uterus implantation mammalian blastocyst stage embryos have already started the process of generating and segregating different cell types required for continued development. Exactly how this remarkable transformation from a single fertilised egg to a highly ordered multi-cellular blastocyst occurs, within just a few days, remains a fascinating and enduring question. During this time embryonic cells either start to adopt specialised functions (differentiate) or they can remain in a so-called ‘pluripotent’ state, only differentiating later in development. Understanding the mechanisms behind such in vivo ‘cell-fate decisions’ is fundamentally important but can also provide insight into the proposed future application of stem cell based regenerative therapies. Research on mouse embryos has begun to address these cell-fate related issues and has highlighted the importance of tight temporal control of specific gene expression and the impact of relative cell position (i.e. on the outside of, or encapsulated within the embryo) to blastocyst formation. Herein, we propose to identify, verify and thoroughly characterise novel early mouse embryo cell-fate influencing genes. Candidate genes have already been identified by integrating our unpublished experimental data aimed at uncovering genes differentially expressed in outer and inner cells with pre-existing datasets describing gene expression throughout the whole of early development. Accordingly, we plan to disrupt the expression of these candidate genes within discreet cell populations of the developing mouse embryo and to observe whether this will affect their ultimate cell-fate. If so, we will determine how this impacts upon already known cell-fate related genes and mechanisms of cell segregation. We will also investigate the consequences of disrupting our validated/ novel cell-fate genes by assessing global effects on gene expression and in turn will yield additional candidate genes for future research.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- medical and health sciences medical biotechnology cells technologies stem cells
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-CIG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MC-CIG - Support for training and career development of researcher (CIG)
Coordinator
370 05 Ceske Budejovice
Czechia
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.