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Content archived on 2024-05-29

Application of photochromic compounds to improve resolution in fluorescence microscopy up to molecular scale

Objective

New techniques in far-field fluorescence microscopy have improved optical resolution down to several times the diffraction limit. The resolution currently achieved with these techniques is 28 nm. Recently it was proposed that fluorescent reversible molecular compounds ('photoswitches') could be utilized in fluorescence microscopy, allowing, in principle, to improve the resolution up to molecular dimensions, i.e. 1-5 nm. The use of these photochromic compounds should enable the utilization of very low light intensities, thus making the technique particularly suitable for biological applications. It is proposed to investigate the feasibility and performance of photochromic fluorescent compounds in modern far-field fluorescent microscopy techniques, as well as explore its different potential uses and applications.

The aim of this project is to improve resolution to a few nanometers and, in particular, apply the technique to live-cell imaging as well as to memory storage and lithography. Undertaking this scientific project in a field leader's laboratory will give me the opportunity to learn advanced microscopy techniques, and acquire a significant experience and expertise in the field. After having completed my training in Germany, I will be prepared to set up a research team and start an independent research career in my home country, Argentina.

Fields of science (EuroSciVoc)

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Keywords

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP6-2002-MOBILITY-7
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

IIF - Marie Curie actions-Incoming International Fellowships

Coordinator

MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
EU contribution
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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