Objective
The ability of the nervous system to respond adaptively relies on modifications to existing proteins as well as changes in gene transcription and protein translation. An individual neuron in the brain possesses approximately 10.000 synapses, many of which are hundreds of microns away from the cell body. As many of the changes to environmental stimuli occur at synapses, the question arises as to how the modified synapses gain access to the new mRNAs and proteins. It is now clear that synapses possess the capacity for local protein synthesis, owing to the localization of ribosomes and mRNAs within dendrites. Previous studies have identified a relative small number of localized mRNAs and an even smaller number of locally synthesized proteins. We hypothesize that synaptic plasticity makes use of the local pool of mRNAs and newly synthesized proteins to alter its’ function. Here we propose to discover the identity of nearly all the mRNAs that are localized, “the local transcriptome”, using the deep RNA sequencing technology. Using a new high-resolution platform that makes use of “fluorescent barcodes” we will visualize individual mRNAs and quantify their abundance in the dendrites. We will also discover the proteins that are synthesized in dendrites, “the local proteome”, using a novel chemical tagging strategy that we developed. In this approach, we can identify proteins synthesized within a given cell-type (e.g. neurons), via the expression of mutant tRNA synthetase in a restricted cell population. We will then examine how plasticity sculpts the local mRNA and protein population, as we hypothesize that both the transcriptome and the proteome are dynamically regulated by ongoing synaptic events.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
ERC-2011-ADG_20110310
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Host institution
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.