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Content archived on 2024-06-18

Transposon based Forward Genetic Screen for the Identification of ncRNAs involved in Colorectal Cancer Metastasis

Objective

In the era of target-based cancer therapies there is still missing a univocal hit for treating cancer metastatic spread, which is ultimately the main cause of cancer death. Non-protein-coding RNA transcripts (ncRNAs) were for long regarded as transcriptional noise; interestingly, it turns out that most of the genome does not carry protein-coding genes (PCGs) and yet is transcribed in a developmentally regulated manner producing ncRNAs with biologically relevant functions (e.g. development and tumor). As for tumor initiation, also for tumor metastasis, it is now becoming common knowledge that the set of metastasis promoting and suppressing genes consists both of PCGs and ncRNAs. Notwithstanding the growing interest in ncRNAs, the ability of forward genetic screens to discover novel genes has not been used in the ncRNA field. Therefore, with the present project we will develop a novel forward genetic screen that specifically retrieves ncRNAs. We will use this genetic screen in human intestinal epithelial cells, which will be tested in anoikis (death induced by anchorage absence) resistance assay. Cells that survive anoikis, which is a rate limiting step during cancer cell metastasis, will have loss of function mutations in ncRNA genes that we will then proceed to characterize.
The present proposal is relevant for the 2011 People Work Programme since it will support the applicant Milena Sabrina Nicoloso in establishing an independent research position in Europe (as a group leader) and ultimately it will improve EU, Italy and the Host Institute excellence thanks to the knowledge and expertise that the applicant acquired during a 5 year international experience in EU competitor countries.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2011-CIG
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-CIG - Support for training and career development of researcher (CIG)

Coordinator

CENTRO DI RIFERIMENTO ONCOLOGICO DI AVIANO
EU contribution
€ 75 000,00
Address
VIA FRANCO GALLINI 2
33081 AVIANO PN
Italy

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Region
Nord-Est Friuli-Venezia Giulia Pordenone
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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