Objective
The rice blast fungus M. oryzae has emerged as a paradigm for the understanding of fungal aerial attack. M. oryzae also infect roots. Consequently, M. oryzae-rice interaction represents an excellent pathosystem for comparative studies of the distinct organ-specific mechanisms that occur during fungal colonisation of rice leaves and roots. We have characterised two pathogenicity-deficient mutants in M. oryzae lacking the karyopherin Msn5p/EXP5 [Plant Cell 22, 953 (2010)] and a novel RNA-binding protein (RBP35) present exclusively in filamentous fungi [PLoS Pathogens, under review]. This project aims to investigate both organ-specific and pos-transcriptional mechanisms that regulate the ability of M. oryzae to infect rice using the resources generated previously in my group. The work outlined in this proposal will extend this area of research through:
1. Deep sequencing experiments using infected rice leaves and roots with a wild-type M. oryzae strain to follow changes in gene expression in the fungus and in the host plant.
2. Tandem affinity purification experiments using EXP5-tagged with HA-FLAG to identify proteins and RNAs that directly interact with EXP5.
3. Tandem affinity purification experiments using highly conserved proteins of the M. oryzae polyadenylation machinery to identify additional protein components and use a combination of SELEX and bioinformatic approaches to discover alternative polyadenylation signals in M. oryzae genes.
4. In vivo visualisation of infection-related mRNAs including fungal effectors using the lambdaN/GFP system to analyse the involvement of RBP35 in mRNA transport and/or localisation.
At present there is a lack of knowledge of the EXP5 cargoes in filamentous fungi and very little is known about the post-transcriptional mechanisms that regulate fungal plant infection. Further, this research will open exciting avenues about local control of translation of proteins significant for M. oryzae plant invasion (fungal effectors).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences microbiology mycology
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-CIG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MC-CIG - Support for training and career development of researcher (CIG)
Coordinator
28040 MADRID
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.