Obiettivo A pivotal question in developmental biology is how cellular gene regulatory networks can respond to a signalling molecule in a concentration-dependent manner. This can now be studied using the modern tools of synthetic biology, an emerging research field that applies engineering approaches to biological systems.This project aims to adapt and develop a band-forming model in E. coli. It will explore systematically the parameter and network space that regulates the formation of a single stripe of gene expression in a morphogen gradient. The full design space of 3-gene networks, where one gene is activated by the morphogen, is 9,710 (isometric networks removed). We are therefore building a flexible network 'scaffold' that will allow the construction of any such circuit and, in particular, the six core topologies predicted in silico to form single stripes.Rational design of gene regulatory networks in vivo is extremely challenging, due to the complex interactions in living cells. Therefore, we are exploring the parameter space that leads to functional band-forming networks by building combinatorial libraries, followed by selection for an appropriate survival pressure.The synthetic networks are engineered with the potential to react as concentration band filters, with respect to a morphogen concentration. The RNA polymerases from the T7 and SP6 phages are used as activators and artificial zinc finger DNA-binding domains, cI and lacI as repressors. The output of the gene regulatory networks is a selectable gene, fused to a green fluorescent protein, which can be used for selection, counterselection and quantification. Campo scientifico natural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesmicrobiologyvirologynatural scienceschemical sciencesinorganic chemistrytransition metalsnatural sciencesmathematicspure mathematicstopologynatural sciencesbiological sciencesdevelopmental biology Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) FP7-PEOPLE-2011-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Invito a presentare proposte FP7-PEOPLE-2011-IEF Vedi altri progetti per questo bando Meccanismo di finanziamento MC-IEF - Intra-European Fellowships (IEF) Coordinatore FUNDACIO CENTRE DE REGULACIO GENOMICA Contributo UE € 168 896,40 Indirizzo CARRER DOCTOR AIGUADER 88 08003 Barcelona Spagna Mostra sulla mappa Regione Este Cataluña Barcelona Tipo di attività Research Organisations Contatto amministrativo Stefan Ponisch (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato