Objective
Toxins occur in a broad diversity in nature and are produced by a wide array of organisms, including spiders, snails, or plants. Conotoxins are a class of toxins that are synthesized in the venom duct of the marine cone snail, genus Conus. The venom of each Conus species is composed of 100 different toxins that rapidly affect the prey organism at multiple targets. The majority of toxins target ligand-gated or voltage-gated ion channels. Each neuroactive peptide of the venom is highly specific for an individual channel isoform.
The applicant proposes to develop in an interdisciplinary approach engineered bacteria that export neuroactive peptides and proteins into the surrounding media, readily available for subsequent characterization. The applicant will utilize the powerful flagellar type-III secretion system of Salmonella enterica for the expression and secretion of recombinant neuroactive peptides. The flagellar-specific type-III secretion apparatus selectively exports substrate proteins at a rate up to 10,000 amino acid residues per second and can be readily manipulated by genetic methods. The applicant will create a Salmonella peptide library based on cDNA isolated from the venom duct of cone snails and use the type-III secretion system as an export shuttle to transport non-flagellar peptides into the surrounding media. The media supernatant or (partially) purified peptides will be analyzed for neurotoxic activity by imaging cellular calcium levels of dorsal root ganglia cells.
This innovative approach for the rapid purification of neuroactive compounds using secretion via the well-characterized flagellar type-III secretion apparatus will allow to identify and characterize novel toxins with distinct target specificity. Due to their unique mode of action and their ability to discriminate between different members of a large ion channel family, these toxins have an immense potential for neurobiological research and novel diagnostic or therapeutic applications.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences microbiology bacteriology
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences organic chemistry amines
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IIF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1700 Fribourg
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.