Objective
The integration of orthogonality is a crucial research strategy in the rational design of biological systems for novel applications. In order to produce such highly predictable orthogonal systems, we propose to employ cell-free systems of highly engineered composition generated from living cells, which are complex enough to reproduce the major synthetic capabilities of living cells - such as the synthesis of natural and artificial saccharides - but are simplified enough to come close to truly engineerable systems. We propose a two-step-strategy: In a first step, the protein synthesis of a growing bacterial cell will be channeled solely to a limited set of system components with the help of the RNA-interferase MazF. In a second step, the cells will be homogenized and the resulting cell-free extract, already enriched in the required protein components, will be subjected to selective hydrolysis of predefined proteins, which otherwise would connect the designed system to the remaining protein background, which would make the performance of the complex system unpredictable. As a proof-of-concept, we propose to implement a preparative 12-step synthesis from cheap glucose and N-acetyl-glucosamine to a valuable antiviral-precursor, N-acetyl-neuraminic acid.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
8092 Zuerich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.