Objective
Two essential phases exist in centromere assembly and maturation during the cell cycle; a G1 phase in which CENP-A is replenished to establish replication competent centromeres and a late-S/G2 phase, in which additional assembly events establish a centromeric substrate competent for kinetochore assembly. The centromere provides a unique context in which to examine the question of epigenetic inheritance. Centromere protein A (CENP-A) is a conserved histone H3 variant that replaces canonical H3 specifically at centromeres in all known eukaryotes. CENP-A, which is manufactured in one cell cycle does not assemble into the centromere until passage through mitosis into telophase of the subsequent cell cycle (Jansen et al., 2007), dependent on a unique factor, HJURP, for deposition and maintenance (Dunleavy et al., 2009; Foltz et al., 2009). A histone H3-associated histone fold complex of the centromere, the CENP-T/W complex, assembles specifically through a dynamic exchange mechanism in late S-Phase and G2 (Prendergast et al., 2011). The CENP-T/-W complex is necessary for successful mitosis (Hori et al., 2008; Prendergast et al., 2011). We hypothesise that assembly factors are involved in the loading of the CENP-T/-W complex to the centromere during S-Phase. To investigate this we have purified soluble pre-deposition CENP-W complexes. Several proteins identified as CENP-W interactors by mass spectrometry, including the FACT complex (SSRP1 / Spt16), have previously been reported as histone chaperones. We hypothesize that one (or more) of these candidates plays a role in the post-replicative preparation of centromeric chromatin for kinetochore assembly, through handling unconventional substrates, the CENP-T/-W complex.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75231 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.