Mechanism of centrosome positioning in HeLa cells

Projektinformationen

Centrosome Position

ID Finanzhilfevereinbarung: 299768

Abgeschlossenes Projekt

Startdatum 1 Juli 2012

Enddatum 29 Dezember 2014

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 167 390,40

EU-Beitrag € 167 390,40

Koordiniert durch

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Germany

Ziel

The Centrosome (CS) is an organelle located near the nucleus that constitutes the primary Microtubule Organization Center (MTOC) in animal cells. The distribution of the microtubule cytoskeleton is therefore determined by the localization of the CS. Localization of this organelle is important for many cellular processes: I) Cell shape and polarization[6], II) Cell migration and III) Cell cycle.

The processes underlying CS localization are mechanical, with two possible scenarios: I) Pushing forces may be generated at the tips of MTs growing from the centrosome against an object such an organelle, cell cortex or plasma membrane; II) Pulling forces may be generated by MTs that remain attach to the CS and their tips shrink against an object. Alternatively, force could be generated along the length or tip of MTs by motor proteins and may be transmitted to the CS. Both pushing and pulling forces have been demonstrated to play a role in different organisms, but how the net force on the CS is regulated in order to achieve proper positioning is still an important open question.

The aim of this project is to study CS positioning in HeLa cells. We will first determine if the dominant force involved in centrosome positioning is pushing or pulling and secondly, explore the different contributions of these forces. To achieve this goal, we will directly perturb the force balance on the CS by severing MTs with laser ablation.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › physical sciences › optics › laser physics

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2011-IEF - Marie-Curie Action: "Intra-European fellowships for career development"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2011-IEF
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IEF - Intra-European Fellowships (IEF)

Koordinator

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV

Kontakt Verwaltung

Birgit Knepper-Nicolai (Dr.)

• EU-Beitrag: € 167 390,40
• Adresse: HOFGARTENSTRASSE 8 ; 80539 Munchen ; Deutschland
• Region: Bayern › Oberbayern › München, Kreisfreie Stadt
• Aktivitätstyp: Research Organisations
• Gesamtkosten: Keine Daten