Obiettivo The interconnections between intermediary metabolism and regulation of gene expression are relatively poorly understood. A few reports have analysed the ability of some metabolic enzymes to 'moonlight' as RNA-binding proteins, being responsive to small metabolites that often represent co-factors of the enzymes. Building on these observations, it was recently proposed that regulatory networks formed between mRNAs, Enzymes and Metabolites (REM networks) might represent a novel regulatory layer interconnecting intermediary metabolism and post-transcriptional regulation of gene expression.This project aimes to comprehensively address the largely unexplored territory of cross-talk between intermediary metabolism and post-transcriptional gene regulation by identifying mRNA-binding proteins in living yeast cells using unbiased proteomic and transcriptomic methods. By adaptation of a protocol established in the host lab for human cells, I will employ in vivo crosslinking and oligo d(T)-purification followed by mass spectrometry to create a catalogue of mRNA-binding proteins for yeast, thereby identifying enzymes conserved between human and yeast. To find 'target' RNAs that specifically interact with these enzymes, I will use crosslinking and immunoprecipitation (CLIP/CRAC) assays followed by high-throughput RNA sequencing. This will allow me to identify the interacting motifs in both, the RNA and the enzymes by bioinformatic and biochemical methods. Finally, I will explore the importance of the mRNA-protein interactions for gene regulation using functional assays in vivo and in vitro. Campo scientifico natural sciencesbiological sciencesgeneticsRNAnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) FP7-PEOPLE-2011-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Invito a presentare proposte FP7-PEOPLE-2011-IEF Vedi altri progetti per questo bando Meccanismo di finanziamento MC-IEF - Intra-European Fellowships (IEF) Coordinatore EUROPEAN MOLECULAR BIOLOGY LABORATORY Contributo UE € 174 475,20 Indirizzo Meyerhofstrasse 1 69117 Heidelberg Germania Mostra sulla mappa Regione Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Tipo di attività Research Organisations Contatto amministrativo Virginia Oton Garcia (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
