Cel Cancer is one of the most common causes of death in the EU. The disease is characterized by aberrant gene activity in certain cells. Even today, cancer therapy is little focused, which is reflected by severe side effects. Consequently, there is a need for novel and better tolerated therapies.In this proposal an approach to validate a new class of enzymes as targets for cancer therapy is presented. In the cell nucleus, DNA is bound to special proteins, called histones, which play key roles in gene regulation. Histones can be dynamically modified with special marker groups, which allows the control of gene expression without alteration of the DNA itself. Specifically, it is intended to inhibit histone demethylases, specialized enzymes which remove certain marker groups (methyl groups) from histones, which results in gene silencing or activation. Histone demethylases are known to be upregulated in certain cancers and are therefore promising candidates for pharmacological cancer therapy. Ideally, small molecule inhibitors will inhibit cancer growth and might even be able to restore the normal genetic program of a cell.During the study specific small molecule-enzyme pairs will be created, which allows the study of a single enzyme among very similar ones. This chemical genetics approach encompasses the mutation of the enzyme and the synthesis of specifically tailored small molecules. The properties of the enzyme-inhibitor pairs will be evaluated by in vitro assays and crystallization. Afterwards the compounds will be used to decipher the role of the histone demethylase in cell culture models by microarray methods and RNA sequencing.The study will be very interdisciplinary and encompass chemical synthesis, biochemistry, cell biology and structural biology. Dziedzina nauki natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencescell biologymedical and health sciencesclinical medicineoncologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesnatural sciencesbiological sciencesmolecular biologystructural biology Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2011-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Zaproszenie do składania wniosków FP7-PEOPLE-2011-IEF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IEF - Intra-European Fellowships (IEF) Koordynator THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Wkład UE € 200 371,80 Adres WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford Zjednoczone Królestwo Zobacz na mapie Region South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Gill Wells (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
