Objective
Cancer is the leading cause of death in the developed world. Significant emphasis has recently been placed on the characterization of the human cancer genome. However, the genetic complexity of cancer has complicated the identification of driver mutations among the more abundant passenger mutations found in tumours. Forward genetic screens are powerful methods to identify genes involved in specific biological processes like cancer formation. However the difficulties and costs currently associated to such screens in vivo in mammalian systems are equally enormous.
Here I propose the creation of a novel site-directed gene transfer system, allowing efficient and controlled somatic gene transfer in vivo in mice. This approach offers the possibility for the rapid creation of large number of somatic clones, over-expressing target proteins, or carrying transgenes to trigger RNAi response against the targeted gene products.
I plan to screen cancer related miR-based gene knockdown libraries in this system, for the identification of novel genes involved in suppressing tumour formation. Recent findings are emphasizing the role of genomic instability caused by errors of DNA repair in the development of cancer. Using candidate gene approach I would like to investigate such mechanisms by the help of the proposed in vivo test system.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- medical and health sciencesmedical biotechnologygenetic engineeringgene therapy
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesgeneticsmutation
- medical and health sciencesclinical medicineoncology
- natural sciencesbiological sciencesgeneticsgenomes
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Call for proposal
FP7-PEOPLE-2011-CIG
See other projects for this call
Coordinator
6726 Szeged
Hungary