Objectif Rhomboids are widely conserved intramembrane proteases. They are known to control important biological processes in model insect, worm, yeast and protozoan species, but their functions in most organisms including mammals and bacteria are unknown. The key to the understanding of rhomboid functions are their natural substrates, but it has been unclear how these are selected and recognized and methods for substrate identification have been lacking, which is limiting progress in the field.The core of this proposal aims to discover the biological roles of highly conserved rhomboids by identifying their natural substrates using a combination of advanced substrate specificity analysis, quantitative proteomics and genetics. I will initially focus on representative conserved bacterial rhomboids and identify their substrate repertoire using an in vitro biochemical screen. Subsequent biochemical and genetic analysis in vivo will reveal the biology of the substrates and rhomboids and indicate functions of their orthologues in pathogens. Enzymatic analysis of the identified substrates will elucidate rhomboid specificity and help us solve the three dimensional structure of a rhomboid substrate complex. In a complementary approach, I will use quantitative proteomics to identify substrate repertoire and elucidate substrate specificity of mammalian endoplasmic reticulum (ER) localised rhomboid RHBDL4, which will enable me to understand its biological role in ER-stress-induced cell death, indicated by preliminary experiments.This project will yield novel biological insights, provide a platform for rhomboid substrate discovery applicable to other biological contexts including pathogens, and provide a deep mechanistic and structural insight into rhomboid protease function, which will help us design new effective rhomboid inhibitors that are needed as experimental tools and have medical potential. Champ scientifique natural sciencesbiological sciencesgeneticsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological scienceszoologyentomologynatural sciencesbiological scienceszoologymammalogy Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2011-CIG - Marie-Curie Action: "Career Integration Grants" Appel à propositions FP7-PEOPLE-2011-CIG Voir d’autres projets de cet appel Régime de financement MC-CIG - Support for training and career development of researcher (CIG) Coordinateur USTAV ORGANICKE CHEMIE A BIOCHEMIE, AV CR, V.V.I. Contribution de l’UE € 100 000,00 Adresse FLEMINGOVO NAM. 542/2 16610 Praha 6 Tchéquie Voir sur la carte Région Česko Praha Hlavní město Praha Type d’activité Research Organisations Contact administratif Jitka Silerova (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
