Objective
RNA silencing pathways exist in most eukaryotes and regulate endogenes to integrate development and environmental responses, and protect organisms against foreign nucleic acids including viruses and transposons. In plants, small interfering RNA (siRNA) and microRNA (miRNA) duplexes of 21-24bp are produced from double-stranded RNA (dsRNA) precursors by Dicer-like RNaseIII enzymes (DCL1-4). Selected si/miRNA strands are then loaded into Argonaute proteins (AGO1-10), which guide an RNA-induced silencing complex (RISC) to mediate sequence-specific post-transcriptional gene silencing (PTGS) at the RNA level, and transcriptional gene silencing (TGS) at the chromatin level. Plant resistance to RNA viruses requires these same components, notably DCL2 and 4 for production of viral-derived siRNAs, AGO1 and 2 to guide viral-targeted RISCs, and an RNA-dependent RNA polymerase (RDR6) and the RNA binding protein SGS3 for amplification of viral dsRNA. While the central role of these factors is well-established, the molecular mode of action that enables these proteins to restrict viral replication is unknown; in fact, the field of RNA silencing is actively studying the extent to which endogenous mRNAs are silenced by AGO-mediated cleavage (“slicing”) or by translational repression (TR) coupled to mRNA decay. In addition, many questions regarding the cell biological and genetic basis for viral silencing have yet to be addressed in a satisfactory manner. This proposal outlines a series of interdisciplinary and mutually-reinforcing experiments to 1) test the mode of action of viral silencing, specifically the relative contributions of slicing and TR during resistance; 2) discover cell biological aspects of viral silencing, particularly where in cells RNA silencing components target viral RNA, and which forms of viral RNA are targeted; and 3) conduct the first large-scale forward genetic screen designed exclusively to reveal components required for viral silencing and its suppression.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences health sciences infectious diseases RNA viruses
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IIF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
8092 Zuerich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.