Objective The thymus plays a central role in the induction of self-tolerance, a hallmark of the immune system. During T cell development a highly diverse T cell receptor repertoire is probed against a matching array of self-antigens. While the generation of the T cell receptor repertoire relies on unique genetic recombination mechanisms, the corresponding molecular principles underlying the generation of TCR ligands - self-peptide/MHC complexes - are less well understood; recent data indicate that novel principles of epigenetic control are employed. This proposal aims at understanding a unique feature of thymic epithelial cells (TECs), namely the expression of a multitude of tissue-restricted antigens (TRAs), a phenomenon termed promiscuous gene expression (pGE) in the context of self-tolerance. The integrative approach of this proposal has 4 main objectives (i) a characterization of the molecular control of different pools of pGE beyond the role of the Autoimmune Regulator (Aire) in relation to the developmental biology of TECs (ii) a comprehensive and comparative analysis of expression patterns of TRAs in single cells of different species in relation to epigenetic signatures and higher chromatin order, (iii) translating these findings to the cellular dynamics and topology of the thymic medullary compartment, e.g. individual mTEC clones in situ (iv) the modelling of these different levels of analysis by a system biology approach, e.g. testing our hypothesis that clusters of TRAs in the genome represent an “operational unit” of pGE. We expect from these studies new basic insights into a fascinating and still arcane aspect of the vertebrate immune system, which will also contribute to our understanding of the etiology and pathophysiology of human autoimmune diseases. Fields of science medical and health sciencesbasic medicinephysiologypathophysiologynatural sciencesmathematicspure mathematicstopologynatural sciencesbiological sciencesdevelopmental biologymedical and health sciencesbasic medicineimmunologyautoimmune diseasesnatural sciencesbiological sciencesgeneticsepigenetics Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) ERC-AG-LS6 - ERC Advanced Grant - Immunity and infection Call for proposal ERC-2011-ADG_20110310 See other projects for this call Funding Scheme ERC-AG - ERC Advanced Grant Coordinator DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG EU contribution € 2 014 560,00 Address Im neuenheimer feld 280 69120 Heidelberg Germany See on map Region Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Activity type Research Organisations Principal investigator Bruno Anton Kyewski (Prof.) Administrative Contact Ina Krischek (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Beneficiaries (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG Germany EU contribution € 2 014 560,00 Address Im neuenheimer feld 280 69120 Heidelberg See on map Region Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Activity type Research Organisations Principal investigator Bruno Anton Kyewski (Prof.) Administrative Contact Ina Krischek (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data