Objective
Methane (CH4) is a potent greenhouse gas produced in diverse terrestrial subsurface and marine ecosystems. However, the amount of CH4 emitted to the atmosphere from these ecosystems is largely reduced by methanotrophic microorganisms –microbes that oxidize CH4 to obtain energy and carbon source for their metabolism. Methane oxidation can occur aerobically or anaerobically. While extensive information is available on aerobic methanotrophs’ biochemistry, physiology and ecology, little is known about anaerobic methanotrophs.
Anaerobic CH4 oxidation has thus far mostly been investigated in marine ecosystems. There, CH4 oxidation is coupled to the reduction of sulfate. It is believed that the reaction occurs via a syntrophic cooperation in which anaerobic methanotrophs transfer electrons to a sulfate reducing bacterium thus the overall reaction is energetically feasible. However, the exact nature of the syntrophy and the identity of the electron transfer compound(s) remain unknown. Moreover, no pure culture or defined consortium of these microorganisms is available. This hampers detailed metabolic and physiology understanding of the process.
Here we propose using innovative bubble-less membrane and electrochemical bioreactors to obtain robust enrichment cultures and to explore the mechanism of electron transfer. The nature of the syntrophy of the microorganisms involved will be assessed using stable isotopes labeled substrates. Incorporation of labeled atoms into biomass –lipids and –fatty acids (LFA) will be determined after cell-sorting of fluorescent labeled various archaea, and bacteria cells types. This new approach may allow exploiting the high sensitivity of stable isotope probing of LFA while attaining good microorganisms’ identity resolution. Metabolomic studies of anaerobic CH4 oxidation will be conducted using in vivo 13C-NMR. The results should result in novel strategies to isolate these not yet isolated key players in the global CH4 cycle.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences microbiology bacteriology
- natural sciences chemical sciences electrochemistry electrolysis
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences biological sciences ecology ecosystems
- natural sciences chemical sciences organic chemistry aliphatic compounds
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75007 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.