Objective
The genetics underlying phenotype-genotype relationships during development and disease is often complex with many genes contributing to a particular phenotypic outcome. While forward genetic screens have uncovered many mutations that are limiting at a particular stage or tissue, the majority of genes in most genomes remain genetically untouched. Recent studies in model organisms, in particular in yeast, have provided evidence for the existence of pervasive genetic interactions with large effects on many phenotypes. Such genetic factors have been rather difficult to identify in classical loss-of-function screens due to buffering and other compensatory mechanisms, therefore requiring novel methodological approaches.
The systematic analysis of synthetic genetic interactions also revealed how biological systems achieve a high level of complexity with a limited repertoire of components. Large-scale studies yeast have taken advantage of deletion strains to construct matrices of quantitative interaction profiles and infer gene function. However, comparable approaches in higher organisms have been difficult to implement in a robust manner.
We here propose to create the first systematic synthetic genetic interaction map of a metazoan cell. Based on methods that we recently pioneered, we will quantitatively measure genetic interactions between genes on a genome scale using Drosophila cells as a model. We will utilize the map to globally dissect the interaction between processes with a focus on signaling networks. Furthermore, we will follow-up on selected interactions by in-depth characterization. Synthetic genetic interactions will be further analyzed in vivo in tissues to complement the cell-based approaches. The project will advance new experimental and computational approaches applicable also to human cells and provide fundamental insights into the cellular circuitries that govern many processes in a metazoan cell.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
ERC-2011-ADG_20110310
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Host institution
69120 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.