Objective
The development of Next Generation Sequencing (NGS) methods has enabled a precise identification of the enteric flora in humans and animals, partly owing to a 16S (bacterial small subunit ribosomal DNA) strategy. However, our insight into eukaryotic endobiotic organisms remains relatively limited. Molecular biological studies of genomic DNA extracted from faecal samples have given us an idea of the diversity among parasites and yeasts present in the human intestinal tract. This knowledge is necessary in order to expand on our understanding of the evolution, ecology and, perhaps most importantly, clinical significance of intestinal eukaryotic organisms, many of which may colonise the host for months or even years, and to evaluate our ability to detect them using current state-of-the-art methods. This insight is, however, still reached primarily by PCR and dideoxy-/Sanger sequencing despite the many limitations of this technology.
To date, there have been no studies employing NGS methods for the investigation of the diversity of endobiotic eukaryotes. This is probably partly due to the fact that a broad 18S strategy – similar to the 16S strategy – involves unwanted amplification of human DNA. However, at the Laboratory of Parasitology, Statens Serum Institut, we have developed an 18S PCR method, which avoids amplification of human DNA. This means that PCR products can be analysed using NGS technology and bioinformatics tools without spending resources on data which have no or little interest. We now wish to validate and use this method to investigate the diversity of the human, eukaryotic enteric flora from different cohorts, including patients with inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), non-IBD/non-IBS diarrhoea and healthy individuals. The results are expected to have a vast impact on the clinical and diagnostic management of intestinal endosymbionts, and to assist in further clarifying the role of these organisms in health and disease.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences genetics DNA
- medical and health sciences health sciences parasitology
- medical and health sciences clinical medicine gastroenterology inflammatory bowel disease
- natural sciences biological sciences ecology
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-CIG
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MC-CIG - Support for training and career development of researcher (CIG)
Coordinator
2300 Kobenhavn S
Denmark
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