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Content archived on 2024-06-18

B-cell development and gene regulation in three dimensions

Objective

A major challenge of modern cell biology is to understand how elaborate transcriptional programs drive developmental processes and maintain cellular identity. In mammals temporal and cell type specific gene regulation depend on regulatory elements in the genome. Genome-wide mapping studies have lead to the identification of myriads of regulatory elements. Strikingly these elements not only exist in abundance compared to genes in the genome, but they are also preferentially located distal to transcriptional start sites in intronic or intergenic regions. Therefore, in contrast to promoters, many regulatory elements can be separated by large genomic distances from their target genes and thus their function can not readily be predicted. A growing body of evidence suggests that regulatory elements exert their function through direct physical interactions mediated by chromatin looping. Thus, in order to accurately describe transcriptional networks and gene-regulation in the frame of B-cell development or indeed in any other cell system, these interactions need to be experimentally determined.
This proposal aims to map interactions between regulatory elements on a genome-wide scale in early B-cell development. Data will be utilized to generating the first transcriptional networks based on interactions between regulatory elements. Further, transcriptional networks will be integrated with transcriptional profiles to validate accuracy of models. Taken together the proposed project should greatly increase our understanding of how transcriptional networks drive developmental progression within the frame of B-cell differentiation.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2012-CIG
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-CIG - Support for training and career development of researcher (CIG)

Coordinator

KAROLINSKA INSTITUTET
EU contribution
€ 100 000,00
Address
NOBELS VAG 5
171 77 STOCKHOLM
Sweden

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Region
Östra Sverige Stockholm Stockholms län
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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