Objective
Recent groundbreaking discoveries have changed our view on RNA from that of a passive information carrier to an important regulatory element. MicroRNA is a small regulatory RNA that controls nearly all mRNAs in eukaryotic cells. Since this regulation process (termed RNA interference/RNAi) occurs in a sequence-specific manner, we can manipulate gene expression using custom-designed small RNAs. This remarkable discovery introduced the possibility of RNA-based gene therapy and triggered intensive research on the RNA-induced silencing complex (RISC), the core machinery of RNAi. The molecular mechanism of RISC is, however, poorly understood due to the limited spatial and temporal resolution of traditional tools, which has deterred development of an RNAi assay applicable to medical sciences.
I will use single-molecule fluorescence to investigate the entire process of RISC action with high spatio-temporal resolution. From ‘RISC assembly’ through ‘target mRNA search’ to ‘target mRNA degradation,’ it requires the cooperative action of multiple RISC components. As the protein-protein and protein-RNA interactions are dynamic processes, it is challenging to study them in bulk where the interactions are diffusion-limited and subsequent processes are masked from observation. With single-molecule microscopy, I will observe all the processes in real time and quantitatively examine the kinetics. In addition, I will dissect the complex processes of RISC action by observing multiple RISC components simultaneously, using multicolor FRET that I have developed. Furthermore, to elucidate the complex nature of RNAi, I will reconstitute protein complexes with a single-molecule immunoprecipitation technique that I have recently innovated.
This first single-molecule study on RISC will enable us to reveal novel molecular mechanisms of RNAi. The fruitful outcome will aid the development of RNAi free from off-target interactions, which will lead to RNAi-based gene therapy in the near future.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
ERC-2012-StG_20111109
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Host institution
2628 CN DELFT
Netherlands
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