Objectif There are several bottlenecks that hinder certain aspects of proteomics, in particular, incompatibility of high throughput technologies with certain protein types or modifications, low sensitivity and lack of quantitative data. I have developed a microfluidics affinity assay compatible with transmembrane proteins and post-translational modifications that is highly sensitive and can provide quantitative data.The primary objective of this proposal is to bioengineer, using the abovementioned building blocks, a multi-functional microfluidic-based human protein arrays. The platform will enable addressing important scientific questions not otherwise possible. Specifically, the process of DNA demethylation, which is poorly characterised due to technological limitations. The biological aspects of chromatin methylation and their regulators that are crucial for cell differentiation and disease will be studied.Work in MuDLOC will include the following: i) Bioengineering of a microfluidic-based platform that expresses thousands of human genes; ii) Design new tools for post-translational modifications and chromatin modifications; iii) Search for chromatin modifiers and their regulators; and iv) Exploration of specific inhibitors using a microfluidic inhibitor screen.Beyond studying chromatin methylation from a new perspective, MuDLOC will greatly benefit a plethora of disciplines, such as proteomics, genomics and cancer research. At the end of the project my vision is to capture under one platform a whole pathway, including protein interactions, post-translational modifications and chromatin modifications. Champ scientifique natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesgeneticsDNAengineering and technologyother engineering and technologiesmicrotechnologylab on a chipmedical and health sciencesclinical medicineoncology Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-SG-LS9 - ERC Starting Grant - Applied life sciences and biotechnology Appel à propositions ERC-2012-StG_20111109 Voir d’autres projets de cet appel Régime de financement ERC-SG - ERC Starting Grant Institution d’accueil BAR ILAN UNIVERSITY Contribution de l’UE € 1 497 990,00 Adresse BAR ILAN UNIVERSITY CAMPUS 52900 Ramat Gan Israël Voir sur la carte Type d’activité Higher or Secondary Education Establishments Chercheur principal Doron Gerber (Dr.) Contact administratif Estelle Waise (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire BAR ILAN UNIVERSITY Israël Contribution de l’UE € 1 497 990,00 Adresse BAR ILAN UNIVERSITY CAMPUS 52900 Ramat Gan Voir sur la carte Type d’activité Higher or Secondary Education Establishments Chercheur principal Doron Gerber (Dr.) Contact administratif Estelle Waise (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
