Objectif Microfluidic systems have a tremendous potential for the miniaturization and automation of bio-chemical assays. Cells or genes can be encapsulated in droplets that are manipulated, fused, analysed and sorted at high-throughput. Such systems are extremely powerful for the selection of microorganisms. To go further, I propose now to develop new types of microreactors for biotechnological assays, combining microfluidics and encapsulation. I propose to generalize droplet production to other encapsulation procedures based on SOFt-Interfaces (surfactant-laden interfaces, particle-laden interfaces, soft polymer shells, biofilms...). First, I will characterize the mechanical properties of soft interfaces using interfacial rheology methods and I will develop novel microfluidic methods for the quantitative measurements of the mechanics of microcapsules. Next, I will use microfluidics as a tool to characterize the chemical stability of the microreactors and to induce the release of the contents of the capsules by external forcing. Finally, I propose to use these new types of microreactors in order to control the adhesion of cells inside the microcontainer to develop new high-throughput screening systems for adherent cells. I also envision a novel type of self-sorting microreactor. Based on the recent developments of self-propelled droplets, I will design a system where the droplets containing the object of interest (for example a cell) would sort themselves, by controlling droplet propulsion with an enzymatic reaction. Sorting of microorganisms based on a specific activity is of considerable interest for applications in diagnostics, or selection of organisms for specific tasks. Such self-sorting microreactors would have a huge potential as a new approach to select efficient microorganisms in an automated manner. Such systems can also be seen in the frame of synthetic biology as new microorganisms capable of motion which can be assembled in a bottom-up approach. Champ scientifique natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsnatural sciencesbiological sciencessynthetic biologysocial sciencessociologyindustrial relationsautomationnatural scienceschemical sciencespolymer sciencesnatural sciencesbiological sciencesmicrobiology Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-SG-PE4 - ERC Starting Grant - Physical and Analytical Chemical sciences Appel à propositions ERC-2012-StG_20111012 Voir d’autres projets de cet appel Régime de financement ERC-SG - ERC Starting Grant Institution d’accueil CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Contribution de l’UE € 1 157 562,92 Adresse RUE MICHEL ANGE 3 75794 Paris France Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Chercheur principal Jean-Christophe Baret (Dr.) Contact administratif Gaëlle Bujan (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (2) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS France Contribution de l’UE € 1 157 562,92 Adresse RUE MICHEL ANGE 3 75794 Paris Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Chercheur principal Jean-Christophe Baret (Dr.) Contact administratif Gaëlle Bujan (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Participation terminée Allemagne Contribution de l’UE € 340 437,08 Adresse HOFGARTENSTRASSE 8 80539 Munchen Voir sur la carte Région Bayern Oberbayern München, Kreisfreie Stadt Type d’activité Research Organisations Contact administratif Theo Geisel (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
