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Pulsed Dipolar Spectroscopy on Symmetric Oligomers

Objectif

Pulse EPR distance measurements have evolved to a standard tool for generating long-range constraints for structural modelling via pulsed dipolar spectroscopy. Especially the pulsed electron−electron double resonance (PELDOR or DEER) method is becoming increasingly applied. The method is particularly promising for structural studies on different functional states of membrane proteins which are difficult to access by most biophysical methods.
However, in samples of membrane proteins several approximations commonly made in data analysis might not be well met. The effect of the finite sample concentration is not necessarily as straightforward as in homogeneous solutions. Furthermore, systems of singly-labelled monomers forming trimers or higher oligomers not only allow extracting the oligomerisation state from the modulation depth, but also exhibit multi-spin effects hampering the distance analysis which are often neglected. These multi-spin effects should increase significantly with oligomeric state.
In the proposed project we will address these problems and develop and validate alternative approaches to data acquisition and analysis in symmetric multi-spin systems.

Appel à propositions

FP7-PEOPLE-2012-CIG
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Coordinateur

THE UNIVERSITY COURT OF THE UNIVERSITY OF ST ANDREWS
Contribution de l’UE
€ 100 000,00
Adresse
NORTH STREET 66 COLLEGE GATE
KY16 9AJ St Andrews
Royaume-Uni

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Région
Scotland Eastern Scotland Clackmannanshire and Fife
Type d’activité
Higher or Secondary Education Establishments
Contact administratif
Trish Starrs (Ms.)
Liens
Coût total
Aucune donnée