chr_lncRNAProject reference: 328577
Funded under :
Identification and functional characterization of lncRNA-chromatin protein complexes associated with specific chromatin modifications in breast cancer
Total cost:EUR 216 952,8
EU contribution:EUR 216 952,8
Topic(s):FP7-PEOPLE-2012-IEF - Marie-Curie Action: "Intra-European fellowships for career development"
Call for proposal:FP7-PEOPLE-2012-IEFSee other projects for this call
Funding scheme:MC-IEF - Intra-European Fellowships (IEF)
It is now know that the mammalian genome encodes many thousands of long non-coding RNAs (lncRNAs, >200nt long), some of which are implicated in human pathogenesis including cancers. A subset of lncRNAs such as HOTAIR and ANRIL are overexpressed in several cancers and play roles in epigenetic gene regulation during carcinogenesis, mainly by recruiting chromatin modifier complexes to their target loci. Interestingly, recent studies indicate that some lncRNAs including ANRIL bind to and act together with other chromatin binding proteins (CBPs) that recognize specific histone modifications. However, lncRNA research is still in its infant stage and physical and functional links among lncRNAs, CBPs and histone modifications have not yet been studied extensively. Moreover, considering the facts that the levels of some CBPs change during carcinogenesis, it is likely that different repertoires of lncRNAs-CBP complexes exist in normal and cancer cells.
Based on these facts, I hypothesize that cell type-specific repertoires of lncRNA-CBP complexes lead to altered readouts of specific histone modifications and ultimately contribute to formation/progression of cancer. Using breast cancer cell lines as model systems, I aim to identify repertoires of lncRNA-CBP complexes associated with cancer-related histone modifications (methyl-H3Lys9/27) in normal and cancer cells and unravel their oncogenic or tumour suppressor functions in vivo using proteomics, genomics, molecular and cell biology approaches. In particular, I will develop a novel, highly versatile method for efficient identification of multiple lncRNA-CBP complexes, by combining photo-mediated RNA crosslinking to proteins, nucleosome-based pulldown and quantitative SILAC mass spectrometry. I believe that the outcome of this project will provide novel and deeper insights into the interplay among lncRNAs, CBPs and histone modifications in carcinogenesis, which can be applied to translational medicine in the future.
EU contribution: EUR 216 952,8
Tel.: +49 551 2011340
Fax: +49 551 2011337