Cel Nicotinic acetylcholine receptors (nAChRs) containing the alpha6 subunit have been implicated in neurological and psychiatric conditions including Parkinson’s and nicotinic dependence. Despite their potential therapeutic importance, little is known about the biophysical properties of these receptors in part because of their restricted tissue distribution and poor expression in heterologous systems. Nevertheless, a recent report by Dr. Albillos’ laboratory demonstrated that the alpha6beta4 subtype is the main nAChR expressed in human adrenal chromaffin cells. This seminal report constitutes the only functional demonstration of native human alpha6beta4 nAChRs and represents a timely opportunity to gain valuable insights into its biophysical properties and to further our understanding of this receptors involvement in the stimulus-secretion response of chromaffin cells. This proposal intends to characterize the biophysical properties of native human alpha6beta4 nAChRs, their interaction with mitochondria via calcium, and their functional regulation by reactive oxygen species (ROS) produced by mitochondria under pathological conditions. The specific aims of this proposal are: 1) to characterize the alpha6-containing nAChR subtype(s) present in human adrenal chromaffin cells and to investigate the regulation of their expression by nicotine; 2) to study the functional interaction between alpha6beta4 nAChRs and mitochondria; 3) the regulation of this nAChR subtype by mitochondrial ROS. Dziedzina nauki natural scienceschemical sciencesinorganic chemistryalkaline earth metalsmedical and health sciencesbasic medicineneurologyparkinson Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2012-IIF - Marie Curie Action: "International Incoming Fellowships" Zaproszenie do składania wniosków FP7-PEOPLE-2012-IIF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IIF - International Incoming Fellowships (IIF) Koordynator UNIVERSIDAD AUTONOMA DE MADRID Wkład UE € 166 336,20 Adres CALLE EINSTEIN 3 CIUDAD UNIV CANTOBLANCO RECTORADO 28049 Madrid Hiszpania Zobacz na mapie Region Comunidad de Madrid Comunidad de Madrid Madrid Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Mª Carmen Puerta (Mrs.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
