Objective
"Coordinated migration of cells is an important process in development and physiology. Collective migration has been extensively studied in tightly connected cell sheets, or epithelia, with recent studies reporting forces in epithelial collective migration. Although a physical and functional connection between the cells throughout the whole process has been thought necessary for collective migration, collective behaviour has recently been reported in the migration of loosely coherent neural crest (NC) cells. The biomechanical basis of this collective behaviour is poorly understood. NC is a classic example of mesenchymal cells: after undergoing an epithelial-to-mesenchymal-transition (EMT) NC cells migrate long distances as a group in a coordinated and cooperative fashion. Although NC's do not form stable connections, they establish short lived contacts. We will test the hypothesis that these temporary contacts are able to transmit sufficient mechanical forces between the cells to create a collective migration pattern similar to epithelia.
We propose to quantitatively describe the collective motion of Xenopus NC cells and the physical forces involved. Mesenchymal behaviour will be compared with the migration of placode and epiderm, two well-known epithelial tissues. We propose to investigate shear and normal stress within the migrating mesenchymal and epithelial groups.
Measurements in vitro will include trajectory analysis, traction forces and intercellular forces, using time lapse, force gel and monolayer stress microscopy. In vivo force generation between NC's will be measured in Xenopus and zebrafish embryos using a FRET mechanosensor, trajectory analysis will be performed in Xenopus graft embryos and transgenic zebrafish line expressing nuclear-RFP in NC cells. Based on experimental findings, the role of transient cell-cell adhesions in collective migration will be studied with cell-based modelling."
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences physical sciences optics microscopy
- medical and health sciences basic medicine physiology
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-IEF
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WC1E 6BT LONDON
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.