Objectif "Luminescence is an attractive strategy for a low cost, real-time and unambiguous detection of cancer cells and tumors due to its high sensitivity and the versatility of the instrumentation. The actual bottleneck is related to the imaging agents whose performances directly control the sensitivity and the selectivity of the real-time detection in living biological systems. Lanthanide luminescence offers several important advantages over organic fluorophores and semiconductor nanocrystals: (i) sharp emission bands for spectral discrimination from autofluorescence and multiplex detection, (ii) emission in the near-infrared, (iii) long luminescence lifetime for temporal discrimination, and (iv) strong resistance to photobleaching. The main idea of this proposal is to create and use new dendrimer lanthanide-based luminescent imaging agents to establish a novel modality of in vivo cancer cells and tumor detection. The proposed work includes the design of luminescent dendrimer complexes, the detailed investigation of their spectroscopic properties, as well as the in vivo tests at the cellular (microscopy) and small animals (macroscopy) levels to evaluate the performances of these imaging agents for practical applications. Proposed unique dendrimer technology is a versatile tool allowing (i) the enhancement of detection sensitivity since many lanthanide ions and lanthanide sensitizers can be combined within one discrete molecule increasing the number of photons per unit volume, (ii) the attachment of a broad variety of lanthanide sensitizers, including those allowing low energy excitation that is less harmful for biological systems, and (iii) the conjugation with a large variety of peptides for selective cancer cells and tumor targeting. Overall, successful completion of this project will result in creation of a novel lanthanide-based imaging methodology allowing real-time, selective and sensitive detection of different types of cancer tumors on the early stages." Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculessocial sciencessociologysocial issuessocial inequalitiesmedical and health sciencesclinical medicineoncologynatural sciencesphysical scienceselectromagnetism and electronicssemiconductivitynatural sciencesphysical sciencestheoretical physicsparticle physicsphotons Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2012-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Appel à propositions FP7-PEOPLE-2012-IEF Voir d’autres projets de cet appel Régime de financement MC-IEF - Intra-European Fellowships (IEF) Coordinateur CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Contribution de l’UE € 194 046,60 Adresse RUE MICHEL ANGE 3 75794 Paris France Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Contact administratif Camille Sèze-Goismier (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
