Skip to main content
Go to the home page of the European Commission (opens in new window)
English English
CORDIS - EU research results
CORDIS
Content archived on 2024-06-18

Combined Selective Plane Illumination Microscopy and Optical Projection Tomography for in vivo quantitative imaging of the developing zebrafish vasculature

Objective

"Recently conceived three-dimensional microscopy techniques are revolutionizing developmental biology and embryology studies, being able to visualize entire organisms in-vivo. Despite increasingly proposed imaging solutions, methods suitable to quantify dynamic and mechanical parameters over a large region of a biological specimen are still lacking. This proposal aims to construct an optical system and a computational framework able to quantitatively study the developing embryonic zebrafish vasculature, looking at the interaction between gene expression and hemodynamic parameters, at high spatial resolution, over a large volume of the organism. The instrument will exploit an advanced version of Optical Projection Tomography, in order to provide a detailed three-dimensional map of the zebrafish cardiovascular system annotated with blood flow and shear stress parameters. Co-registered Selective Plane Illumination Microscopy images will enable the users to observe the expression of fluorescent markers at sub-cellular resolution and to correlate it to the hemodynamic/mechanical parameters. Low photo-bleaching and high acquisition speeds make this multimodal technology ideally suited for extended time-lapse experiments in live embryos. The system will be employed to characterize the development of the embryonic cardiovascular system in normal and mutant zebrafish embryos.
The researcher, proponent of this project, will be integrated in a highly multidisciplinary team and will be trained on microscopy and biological aspects of the research, being able to acquire new skills relevant for the development of his optical tomography laboratory and his career."

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

You need to log in or register to use this function

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2012-IEF
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
EU contribution
€ 84 397,20
Address
HOFGARTENSTRASSE 8
80539 MUNCHEN
Germany

See on map

Region
Bayern Oberbayern München, Kreisfreie Stadt
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

No data
My booklet 0 0