Objective
Regulation of RNA stability is recognized as a widely used strategy to regulate protein abundance in time and space in all eukaryotes. RNA degradation regulates gene expression in various processes during development and differentiation and in changing physiological conditions. Many elements of the RNA decay machinery have been identified, but details of their function remain elusive.
The exosome is a multi-subunit exonuclease complex that functions in turnover, quality control and processing of many RNA species. Depending on its subcellular localization it requires different accessory protein complexes that specifically target RNAs towards the exosome and stimulate the degradation process. In the yeast cytosol the Ski complex is the major accessory factor of the exosome. It is required for nearly all exosome-mediated mRNA degradation processes. The core Ski complex is formed by Ski2, an RNA helicase, the large TPR protein Ski3 and two copies of Ski8. This core complex interacts genetically and physically with the exosome via the GTPase Ski7.
In the proposed project, I will study how the activities of the Ski238 complex, Ski7p and the exosome are concerted. For this I will recombinantly express, purify and crystallize the Ski2387 complex alone and together with the exosome to learn about their interaction on atomic level. This approach structural approach will be complemented by electron microscopic studies of the complexes. The results of the structural analysis will guide the design of subsequent functional biochemical and in vivo studies.
The study will be exemplary for the understanding of the functioning of an important cellular machinery in atomic detail.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.