Objective
Nucleoporins are major building blocks of the nuclear pore complex (NPC). In addition to their role in the nucleocytoplasmic transport, a subset of nucleoporins have additional gene regulatory roles. They can bind chromatin and chromatin modifiers, either directly at the NPC or in the nucleoplasm, leading to gene repression or activation. Due to a lack of techniques that allow mapping spatial and temporal heterogeneities of individual proteins with high precision, the molecular mechanisms underlying these phenomena are far from being understood. To overcome this, I will develop a combined protein-engineering/super-resolution approach for single amino acid targeted labelling and tracking of proteins inside the cells. This strategy is based on the translational incorporation of newly developed non-canonical amino acids (ncAAs). These ncAAs are suitable for inverse-electron-demand Diels-Alder reaction, a special type of highly selective and rapid “click-chemistry” reactions proceeding in a non-toxic and fully biocompatible way inside living cells. I will employ this technology to systematically label a set of nucleoporins and chromatin modifiers with small dyes. The high photostability of these fluorophores permits long-term imaging and will allow me to identify nucleoporin binding partners in cultured mammalian cells with nanometer resolution. This will set the stage for a technology that enables deciphering protein plasticity in vivo at the molecular level and provide novel insights into multifunctionality of nucleoporins.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences organic chemistry amines
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.