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Content archived on 2024-06-18

Dynamic Microfluidic Deflection Systems for High Throughput Single Platelet Function Testing

Objective

"Single activated platelets can activate other platelets to drive blood clotting. Single, hyper-sensitive platelets could coordinate clotting in healthy individuals or cause pathological clotting in high risk groups such as the elderly, obese peoples and smokers. To identify potentially rare hypersensitive platelets, an assay must enable high throughput population profiling with all platelets isolated to avoid cross-talk. Platelet activation is associated with a <5 second transformation from a discoid to a stellate morphology and with zeta-potential changes caused by membrane inversion. However, heterogeneity within the population prevents the fractionation of hypersensitive platelets using size and charge based deflection fields. The proposed research aims to solve these challenges using bespoke microfluidic platforms integrating a biochemical switch with deterministic lateral displacement or free-flow electrophoresis deflection fields for imaging platelet trajectories DURING activation. Critically, TRAJECTORY CHANGES, consistent with platelet reorganisation dynamics, are DIAGNOSTIC for ACTIVATION and the continuous flow format generates a single-file platelet stream, effectively isolating platelets from one another. Effective size, shape and charge components of deflection will be elucidated to provide new insights into platelet activation dynamics. Platelets from healthy and high risk cohorts will be screened to identify hyper-sensitive populations and determine therapeutic doses required to desensitise the hypersensitive platelets. This multidisciplinary research will create a paradigm shift, from a population to a single platelet perspective. This discovery to diagnostics work program has great potential to generate intellectual property to underpin commercialization of a point of care diagnostic for life-long health care in the EC. Outputs from this research will also feedback to identify new challenges and opportunities in separation science."

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2012-CIG
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-CIG - Support for training and career development of researcher (CIG)

Coordinator

UNIVERSITY OF SOUTHAMPTON
EU contribution
€ 100 000,00
Address
Highfield
SO17 1BJ Southampton
United Kingdom

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Region
South East (England) Hampshire and Isle of Wight Southampton
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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