Objectif During the last five years, within the EU FP6 Integrated Project XENOME (LSHB-CT-2006-037377), we have convincingly demonstrated in primates that a complete control of induced type 1 diabetes is routinely obtained for 6/8 months after transplantation of subcutaneous alginate macroencapsulated porcine islets xenograft without the use of any immunosuppression. In order to improve the function of pig islets, we have produced with AVANTEA a transgenic pig expressing GLP-1 at the level of the pig islets. In fact, GLP1 is not only able to (i) increase the insulin gene expression and insulin biosynthesis, but also to (ii) induce the replication of islet cells and promotes islet-cell neogenesis and (iii) protect islets cells from apoptosis. GLP-1 has already demonstrated its in vivo potential to improve insulin secretion in subjects with impaired glucose tolerance and type 2 diabetes. New vectors (GLP1/M3Rr) containing both GLP1 sequence and muscarinic receptor mutation (M3R) will also be tested. The main objectives of this four-year project will therefore be (1) to evaluate in vitro the effect of GLP1/M3R transgene expression in pig islets after hyperglycaemic challenge; (2) to continue to produce double transgenic pigs specifically expressing GLP-1/M3R in pig islets under insulin promoter, and (3) finally to test these modified pig islets in our well characterized pig-to-diabetic primates model in vivo. However, in order to use islets from newborn unmodified or transgenic pigs, we will also set up (4) the pig islets isolation and cell maturation technique for Neonates Pancreatic Pig Cluster cells since neonates pig islets have prolonged survival and would also render possible the use of Designed Pathogen Free (DPF) neonates pigs which will represent a key point to reach clinical studies. BIOT will be mainly involved in this part of the work which is in vitro purification, amplification of NPCCS. GCU and AVIDIN Ltd will together develop arrays and a set of molecular testing for pig cell pathogens.A pilot study for safety will be achieved whether all the prerequisite are achieved at the end of the fourth year project. Champ scientifique natural sciencesbiological scienceszoologymammalogyprimatologymedical and health sciencesclinical medicineendocrinologydiabetesnatural sciencesbiological sciencesgeneticsmutationmedical and health sciencesclinical medicinetransplantation Programme(s) FP7-HEALTH - Specific Programme "Cooperation": Health Thème(s) HEALTH.2013.0-1 - Boosting the translation of health research projects' results into innovative applications for health Appel à propositions FP7-HEALTH-2013-INNOVATION-2 Voir d’autres projets de cet appel Régime de financement CP-FP - Small or medium-scale focused research project Coordinateur UNIVERSITE CATHOLIQUE DE LOUVAIN Contribution de l’UE € 1 641 678,89 Adresse PLACE DE L UNIVERSITE 1 1348 Louvain La Neuve Belgique Voir sur la carte Région Région wallonne Prov. Brabant Wallon Arr. Nivelles Type d’activité Higher or Secondary Education Establishments Contact administratif Pierre Gianello (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire THE GLASGOW CALEDONIAN UNIVERSITY Royaume-Uni Contribution de l’UE € 731 660,00 Adresse Cowcaddens Road, City Campus 70 G4 0BA Glasgow Voir sur la carte Région Scotland West Central Scotland Glasgow City Type d’activité Higher or Secondary Education Establishments Contact administratif Linda Scobie (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée AVANTEA SRL Italie Contribution de l’UE € 914 500,00 Adresse VIA CABRINI 12 26100 CREMONA Voir sur la carte Région Nord-Ovest Lombardia Cremona Type d’activité Private for-profit entities (excluding Higher or Secondary Education Establishments) Contact administratif Giovanna Lazzari (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée BIOTALENTUM TUDASFEJLESZTO KFT Hongrie Contribution de l’UE € 971 000,00 Adresse AULICH LAJOS UTCA 26 2100 Goedoello Voir sur la carte Région Közép-Magyarország Pest Pest Type d’activité Private for-profit entities (excluding Higher or Secondary Education Establishments) Contact administratif Andras Dinnyes (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée AVIDIN KUTATO, FEJLESZTO ES KERESKEDELMI KFT Hongrie Contribution de l’UE € 778 309,11 Adresse ALSO KIKOTO SOR 11 6726 SZEGED Voir sur la carte Région Alföld és Észak Dél-Alföld Csongrád Type d’activité Private for-profit entities (excluding Higher or Secondary Education Establishments) Contact administratif Laszlo Puskas (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
