Objective
The polarisation of neurons in somatodendritic and axonal compartments allows signal propagation over long distances. Despite the important role played by electrical signals, fast axonal transport is crucial for long-range communication between the soma and distal synapses, which is carried out by vesicular transport mechanisms. Neurotrophins and their receptors are internalised at axon terminals by clathrin-mediated endocytosis and conveyed by signalling endosomes to the soma, where they activate transcriptional responses regulating neuronal homeostasis and survival. However, little is known about the identity and the dynamics of the organelles involved.
The aim of my project is to build a functional and physical map of axonal retrograde carriers in specific neuronal types, which are the target of important human diseases. For this purpose, I will exploit a new developed affinity purification strategy using the binding fragment of tetanus neurotoxin (HC) conjugated to monocrystalline iron oxide nanoparticles (MIONs).
At different time points after internalisation, retrograde carriers will be magnetically purified and submitted to mass-spectrometry analysis. The kinetics of endosomal maturation will be assessed using quantitative label-free SILAC protocols. These results will uncover new components of signalling endosomes and identify dysfunctions of this pathway that are at the basis of motor and sensory neuropathies.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- engineering and technology nanotechnology nano-materials
- medical and health sciences basic medicine physiology homeostasis
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WC1E 6BT LONDON
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.