Objective
The long-term goal of this proposal is to understand the molecular mechanisms of the replication cycle of
nucleocytoplasmic large DNA viruses that attracted attention after the discovery of the giant Mimivirus a
decade ago. Here, we focus on ranaviruses from the family Iridoviridae that have caused large economic
losses in Europe and Asia and threaten wildlife biodiversity worldwide. The ranavirus disease has therefore
been listed by the World Organization for Animal Health. Ranaviruses have a unique replication cycle that
leads to two coexisting forms of infectious particles, naked capsids and enveloped virions, each with
different mechanisms of cell egress and cell entry. Additionally, the proteinaceous capsid contains an
internal membrane that presumably assists to assembly of a large DNA-free icosahedral shell. This shell
is subsequently filled by the viral genome and transforms into the mature capsid by a headful packaging mechanism that is also used by some dsDNA bacteriophages. We aim to characterize the structural and mechanistic features of ranavirus
assembly, maturation and cell entry by combination of cryo-electron microscopy and tomography with other
complementary techniques. The novelty in our plan is application of cryo-FIB micromachining and cryo-electron
tomography to visualize the intracellular steps of the ranavirus replication cycle in 3D at native
conditions. The three objectives of this research project are to: (i) identify the structural basis of subunit
recognition that guides assembly of large icosahedral capsids of type ranaviruses and elucidate the function
of the internal membrane in this process, (ii) determine the conformational changes that accompany
maturation of ranavirus capsids and regulate the headful packaging of the dsDNA genome, and (iii) ascertain
the mechanisms of cell entry by the naked capsids and enveloped ranavirus virions.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences genetics genomes viral genomes
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences biological sciences ecology ecosystems
- medical and health sciences health sciences infectious diseases DNA viruses
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2013-CIG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MC-CIG - Support for training and career development of researcher (CIG)
Coordinator
601 77 Brno
Czechia
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.