Objective
Immune cells constantly receive signalling inputs such as pathogen-emitted molecules, use gene regulatory pathways to process these signals, and generate outputs by secreting signalling molecules like cytokines. Characterizing the input-output relationship of a biological system helps understanding its regulatory mechanisms, and allows building models to predict how the system will operate in complex physiological scenarios, such as population tissue response to infection. A major obstacle in this endeavor has been the so-called “biological noise”, or significant variability in measured molecular parameters between cells. Such variability makes time-dependent single-cell analysis crucial to understand how biological systems operate. Development of new analytical tools with improved functionality, accuracy, and throughput is needed to realize the full potential of single-cell analysis. We propose to develop automated, high-throughput, Optofluidic single-cell analysis systems with unprecedented capabilities, and to use them in understanding how immune cells organize in tissue during response to infection. Microfluidic membrane-valves, nanodroplets, optics, and automation will be integrated to achieve an unparalleled degree of control over single immune cells. Multi-functional lab-on-chip devices will simultaneously measure: a) The activity of immune regulatory proteins such as NF-κB, and b) Inflammatory cytokines secreted from single immune cells in a time-dependent manner, under precisely defined biochemical inputs. Characterizing macrophage cytokine secretion dynamics under combinatorial regiments of bacterial and apoptotic-cell signals will allow dissecting the signalling mechanism responsible from the resolution of inflammation. We will identify the role of the NF-κB pathway in regulation of cytokine dynamics. We will use our data to develop a computer model of tissue-level immune response to pathogens through the NF-κB pathway and cytokine signaling.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- engineering and technology other engineering and technologies microtechnology lab on a chip
- natural sciences biological sciences biochemistry biomolecules proteins
- social sciences sociology industrial relations automation
- medical and health sciences basic medicine immunology
- natural sciences physical sciences optics
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
ERC-2013-StG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Host institution
8092 Zuerich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.