Objective Autophagy is a cellular pathway that regulates the degradation and recycling of proteins and organelles. This mechanism is initiated with the formation of a phagophore, a cup-shaped double membrane that engulfs the cytoplasmic material to be degraded. The phagophore is then elongated and sealed to generate an autophagosome that will be fused with a lysosome, thereby delivering the cargo for degradation. Several ubiquitin-like proteins have been shown to have a crucial role in regulating autophagosome generation and its fusion with the lysosome. One of these proteins is LC3-I, is a soluble cytosolic protein that associates with the autophagosome after C-terminal conjugation to a phosphatidylethanolamine unit (LC3-II). This membrane association is reversibly modulated by the cysteine protease atg4, which deconjugates PE from LC3-II, thus recycling the soluble LC3-I. Detailed studies of the molecular mechanism regulating autophagosome formation and the role of LC3-I and LC3-II are unknown, mainly due to the lack of tools enabling a profound characterization of these processes. Herein, we propose the generation of semisynthetic lipidated LC3 proteins using a chemical biology approach. The fully modified proteins should be unvaluable tools that will contribute to the characterization of the factors regulating autophagy. Fields of science engineering and technologyenvironmental engineeringwaste managementwaste treatment processesrecyclingnatural sciencesbiological sciencesbiochemistrybiomoleculesproteins Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2012-CIG - Marie-Curie Action: "Career Integration Grants" Call for proposal FP7-PEOPLE-2012-CIG See other projects for this call Funding Scheme MC-CIG - Support for training and career development of researcher (CIG) Coordinator AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS EU contribution € 100 000,00 Address CALLE SERRANO 117 28006 Madrid Spain See on map Region Comunidad de Madrid Comunidad de Madrid Madrid Activity type Research Organisations Administrative Contact Ana Maria De La Fuente (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
