Specification of anterior definitive endoderm, the role of extracellular matrix and Akt1 signalling

Projektinformationen

SPECADE

ID Finanzhilfevereinbarung: 327585

Abgeschlossenes Projekt

Startdatum 1 Januar 2014

Enddatum 6 September 2016

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 221 154,60

EU-Beitrag € 221 154,60

Koordiniert durch

KOBENHAVNS UNIVERSITET
Denmark

Ziel

Deciphering the specific role of signalling and the niche in which cells develop and differentiate is one of the central problems of developmental biology. Approaching this problem has been difficult in vivo, as embryonic development involves complex signalling machinery directly instructing the cell fate, or influencing the environment which itself drives the cell specification. Embryonic stem (ES) cell technology affords a new approach, allowing in vitro reconstitution of elements guiding cell differentiation in a defined manner. ES cells can therefore be used to break down developmental decisions and understand the molecular basis of cell fate choice.

In SPECADE, I will use mouse ES cells to analyse the signalling underlying differentiation of anterior definitive endoderm (ADE), the precursor of the ventral foregut and a number of visceral organs including pancreas and liver. I will build on previous work at the hosting Brickman laboratory, which has recently shown an unexpected role of extracellular matrix (ECM) in patterning the definitive endoderm. Revealing, that Fibroblast Growth Factor and coupling PI3K/Akt1 signalling pathways act both in vitro and in vivo to regulate protein level of Fibronectin in the ECM.

The aims of my project are therefore to identify and understand the mechanism by which PI3K/Akt1 signalling modifies the ECM composition and how the specific ECM induces the anterior character in the endoderm. SPECADE’s approach is to study ES cells as a whole system, including its surrounding environment. This will enable us to understand and recapitulate in vivo development, bringing us closer to successful generation of functional cell types in vitro, such as hepatocytes and pancreatic beta cells. This can be used therapeutically for the treatment of diseases such as diabetes and cancer and is one of the main objectives of the current research at the hosting institute, the Danish Stem Cell Center (DanStem).

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › developmental biology
• medical and health sciences › clinical medicine › endocrinology › diabetes
• medical and health sciences › medical biotechnology › cells technologies › stem cells
• medical and health sciences › clinical medicine › oncology

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2012-IEF - Marie-Curie Action: "Intra-European fellowships for career development"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2012-IEF
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IEF - Intra-European Fellowships (IEF)

Koordinator

KOBENHAVNS UNIVERSITET

Kontakt Verwaltung

Bjarne Friis Ploumark (Mr.)

• EU-Beitrag: € 221 154,60
• Adresse: NORREGADE 10 ; 1165 Kobenhavn ; Dänemark
• Region: Danmark › Hovedstaden › Byen København
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten