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Content archived on 2024-06-18

A Total Photon Camera for Molecular Imaging of Live Cells

Objective

"How can we construct a high-resolution camera capable of imaging the time-of-arrival, polarisation and wavelength of each of the maximal 10Gphoton/s emitted from a labelled, biological cell?
Such a measurement would capture the complete information available in the optical signal, and significantly enhance our ability to observe the organisation, movement and interactions of cellular components at molecular scales. Advances in single molecule light microscopy are steadily improving our understanding of the processes underlying normal cellular function, and their alteration in disease states. However, these technologies are unable to reach their full potential due to their reliance on pre-existing, suboptimal detectors. A dedicated camera technology is now required to permit simultaneous, multidimensional measurements of large cohorts of molecules at high temporal and spatial (sub-diffraction limit) scales through total imaging of the photon flux.
Today’s digital cameras capture photons in packets of 10-100 thousand and provide them for external display or recording at fraction of second intervals. In order to process photons individually rather than as packets we must develop a camera operating 10-100 thousand times faster. Each pixel must be capable of capturing single photon parameters without compromising the high resolution and sensitivity achieved by current technology. The ""total photon"" camera will be realised in nanoscale CMOS technology, based on recent breakthroughs in ultra-miniature single-photon detectors. We will combine these with novel approaches to pixel circuits, image processing and high-speed readout electronics to provide a fundamental research tool for the emerging area of computational microscopy. We will provide access to the full record of photon emission from live cells, and hence the clearest possible visualization of dynamic cellular processes in a single device capable of wide-field molecular spectroscopy and superresolution imaging."

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

ERC-2013-ADG
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERC-AG - ERC Advanced Grant

Host institution

THE UNIVERSITY OF EDINBURGH
EU contribution
€ 1 354 862,00
Address
OLD COLLEGE, SOUTH BRIDGE
EH8 9YL Edinburgh
United Kingdom

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Region
Scotland Eastern Scotland Edinburgh
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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Beneficiaries (2)

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