Objectif TO DEVELOP AND EVALUATE AND ECONOMIC, MEDIUM TERM BIOASSAY FOR CARCINOGENICITY BASED UPON TUMOUR RESPONSE. THE PROJECT IS A CONTINUATION AND EXTENSION OF WORK CARRIED OUT FROM 1979 TO 1981 UNDER CONTRACT ENV-323-79-4-I. THE PREVIOUS EXPERIMENTS, PERFORMED ON SPRAGHE-DAWLEY RATS, ARE REPEATED ON SWIS MICE, WHICH HAVE A HIGH LIABILITY TO DEVELOP LUNG ADENOMAS, MAMMARY CARCINOMAS, LYMPHOMAS AND LEUKAEMIAS, UNDER CARCINOGENIC STIMULI. PREGNANT MICE AT 12 DAYS OF PREGNANCY ARE EXPOSED TO THE HIGHEST BEARABLE DOSES OF THE COMPOUNDS UNDER STUDY, ADMINISTERED BY INHALATIONS. THE TREATMENT OF MOTHER LASTS 26 WEEKS. AS IN PREVIOUS EXPERIMENT, THE FOLLOWING COMPOUNDS ARE STUDIED: VINYL CHLORIDE, VINYLIDENE CHLORIDE, ACRYLONITRYLE, BENZENE, METHYLENE CHLORIDE. THE ANIMALS ARE EXPOSED, BY INHALATION; THE LEVEL OF EXPOSURE DURING THE TREATMENT IS MONITORED BY GAS CHROMATOGRAPHY. THE ANIMALS ARE CONTROLLED AND WEIGHED RESPECTIVELY: DURING THE FIRST THREE MONTHS OF THE EXPERIMENTS WEEKLY AND EVERY TWO WEEKS, UP TO 26 WEEKS EVERY TWO WEEKS AND EVERY FOUR WEEKS, AND THEN EVERY TWO WEEKS AND EVERY EIGHT WEEKS. A COMPLETE AUTOPSY IS MADE OF EVERY ANIMAL. HISTOLOGICAL SPECIMENS INCLUDE THE BRAIN, ZYMBAL GLANDS, INTERSCAPULAR BROWN FAT, SALIVARY GLANDS, TONGUE, LIVER, SPLEEN, KIDNEYS, STOMACH, DIFFERENT SEGMENTS OF INTESTINE, BLADDER, LYMPHODES AND EVERY OTHER ORGAN WITH PATHOLOGICAL LESIONS. Programme(s) PRE-ENVPROT 3C - Sectoral research and development programme (EEC) in the field of environment (environmental protection and climatology) - indirect and concerted actions - Environmental protection -, 1981-1985 Thème(s) Data not available Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur Regione Emilia Romagna Contribution de l’UE Aucune donnée Adresse Viale G.B.Ercolani 2/4 40138 Bologna Italie Voir sur la carte Coût total Aucune donnée
