DEVELOPMENT OF BIOCHEMICAL AND IMMUNOLOGICAL ASSAYS FOR DNA RECOMBINATION AND REPAIR

Objetivo

Using affinity purified anti-recA antibodies, we identified a yeast protein which displayed several of the properties expected for a recombinase:
induction after ultraviolet irradiation;
increased expression in concomitance with recombination at meiosis;
nuclear localization.

The protein was partially purified but no activity associated with a recombinative function was detected. Analysis of the sequence of the corresponding gene indicated that the protein showing immunological cross reactivity to recA is the small subunit of ribonucleotide reductase.

Research conducted has been as follows:
purification and characterization of the eukaryotic analogues of recA protein;
development of immunological reagents and analysis of the induction of recA analogues following treatments which damage deoxyribonucleic acid (DNA);
cloning of the genes encoding recA protein analogues in yeast and mammalian cells;
development of a general method for the identification of proteins involved in DNA recombination and repair.
1. PURIFICATION AND CHARACTERIZATION OF THE EUKARYOTIC ANALOGS OF RECA PROTEIN.
2. DEVELOPMENT OF IMMUNOLOGICAL REAGENTS AND ANALYSIS OF THE INDUCTION OF THE RECA ANALOGS FOLLOWING DNA DAMAGING TREATMENTS.
3. CLONING OF THE GENES ENCODING THE ANALOGS OF RECA PROTEIN IN YEAST AND MAMMALIAN CELLS.
4. DEVELOPMENT OF A GENERAL METHOD FOR THE IDENTIFICATION OF PROTEINS INVOLVED IN DNA RECOMBINATION AND REPAIR.

EXPECTED BENEFITS

EVALUATION OF GENETIC RISKS FROM IONIZING RADIATION. DETERMINATION OF CELLULAR RADIOSENSITIVITY AND RELATION TO REPAIR.

Ámbito científico

Programa(s)

FP1-RADPROT 6C - Multiannual research and training programme (Euratom) in the field of radiation protection, 1985-1989

Tema(s)

Data not available

Convocatoria de propuestas

Data not available

Régimen de financiación

CSC - Cost-sharing contracts

Coordinador

Universita Degli Studi Di Milano

Aportación de la UE

Sin datos

Dirección

Via Festa del Perdono 7
20122 Milano
Italia

Coste total