Objectif Saccharomyces cerevisiae was selected as a model system due to its potential industrial interest. In this organism there are 3 acidic proteins present in the ribosome. The proteins were purified and polyclonal and monoclonal antibodies specific for each polypeptide were prepared. The genes encoding ribosomal proteins L44, L45 and L44' were cloned and strains lacking each one of the genes were obtained subsequently using gene disruption techniques. The defective strains were viable. The results have shown that the different acidic ribosomal proteins seem to play a similar role and can be substituted functionally among themselves. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteins Programme(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Thème(s) Data not available Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Contribution de l’UE Aucune donnée Adresse Serrano 117 MADRID Espagne Voir sur la carte Coût total Aucune donnée
