Objetivo AVAILABILITY OF A TOOL FOR THE SOLUTION OF PRACTICAL PROTEIN DESIGN PROBLEMS IN MOLECULAR BIOLOGY, MOLECULAR MEDICINE AND BIOTECHNOLOGY, WITH LONG TERM ECONOMIC BENEFITS IN THESE FIELDS. Data on known protein structures and amino acid sequences have proven to be very useful for deriving empirical rules for protein folding and design. With the growing volume of these data, more sophisticated systems for storing and handlingknowledge on macromolecular structures are urgently needed. Progress should be made by improving ways to exploit sequence homology to infer structural information from the more than 10000 proteins for which only sequence data are available.Research was carried out in order to develop a database of protein knowledge containing structure and sequence information and extend the database to include information on inferred 3-dimensional structures of proteins for which only sequence data are available.SESAM, a performing relational database for protein structure and sequence capable of containing data from public and private sources, was developed; it features powerful procedures for validating and cleaning up input data, and rapid data retrieval. It has been interfaced with a graphics package, BRUGEL, and specialized user friendly interfaces have been implemented. The database on known protein structures was extended to include inferred 3-dimensional structures, grouped into structural families, by exploiting the correlations between structural homology and sequence similarity above a certain threshold of the latter. A limited number of short sequence patterns characterising with high accuracy local structure motifs in proteins can be found. This does not improve protein structure prediction methods, due to the limited size of the structural database and to the influence of spatial interactions between distant residues in the sequence. Object oriented methods and logic programming (Prolog) yield important benefits in terms of speeding up the design, development and debugging stages.DEVELOPMENT OF A SYSTEM FOR PROTEIN STRUCTURE PREDICTION, MERGING STATISTICAL AND INFORMATION ANALYSIS TECHNIQUES WITH ADVANCED TOOLS FROM COMPUTER SCIENCE (A.I.) AS WELL AS EXISTING METHODS IN MOLECULAR MODELLING. IN THIS COLLABORATIVE EFFORT, THE SPECIFIC ROLE OF THIS PART OF THE PROJECT CONSISTS IN THE DEVELOPMENT OF THE REQUIRED COMPUTER TOOLS. DEFINITION AND IMPLEMENTATION OF LOGIC BASED REPRESENTATION SCHEMES (IN COLLABORATION WITH THE GROUPS A - C) OF RESOURCE EFFICIENT META-INTERPRETERS AND USER INTERFACES. Ámbito científico natural sciencescomputer and information sciencesartificial intelligencenatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsprotein foldingnatural scienceschemical sciencesorganic chemistryaminesnatural sciencescomputer and information sciencesdatabasesrelational databasesnatural sciencesbiological sciencesmolecular biology Programa(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador BELGIAN INSTITUTE OF MANAGEMENT Aportación de la UE Sin datos Dirección KWIKSTRAAT,4 3078 EVERBERG Bélgica Ver en el mapa Coste total Sin datos Participantes (4) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo EMBL Alemania Aportación de la UE Sin datos Dirección HEIDELBERG Ver en el mapa Coste total Sin datos INSTITUT PASTEUR Francia Aportación de la UE Sin datos Dirección Rue du Docteur Roux 25 75724 PARIS Ver en el mapa Coste total Sin datos SUBCONTRACT : KUL Bélgica Aportación de la UE Sin datos Dirección HEVERLEE Ver en el mapa Coste total Sin datos ULB Bélgica Aportación de la UE Sin datos Dirección BRUXELLES Ver en el mapa Coste total Sin datos
