Cel THE LONG RANGE GOAL IS TO OBTAIN THE KNOWLEDGE NECESSARY FOR INTRODUCING APPROPRIATE CHANGES IN ENZYMES OF INDUSTRIAL INTEREST. How changes in a protein's structure modify its functions. The approach was based on research into insulin and the enzymes amylase and lipase, the goal being to determine the structure of the proteins and the modified proteins and the modified proteins by X-ray crystallography. With this technique, the position of the modified groups in insulin and the enzymes and their interactions with the surrounding proteins can be accurately determined and related to changes in function. This detailed structural information is essential for designing new changes in function of insulin and the enzymes. New technologies were also developed for increasing the quality of the data from X-ray experiments.The 3-dimensional structures of mutated insulins were determined and the role the changed amino acid side chains had in the modified properties of the hormone was assessed. Insulin's properties of self assembly are best affected by introducing new charges which repel each other when the individual molecules come together. The structures of 2 amylases were determined, revealing details of their catalytic sites. Calcium ions were developed to bind at the catalytic groups and thus abolish enzymatic activity. One of the 2 enzymes is active in acid conditions. The 2 structures are now being compared. Finally, the crystal structure of a fungal lipase was also solved. This showed that the catalytic group in this enzyme is very similar to the catalytic group in the serine proteases.THE INDUSTRIAL ENZYME OF INTEREST WILL BE PURIFIED, CHARACTERIZED AND SEQUENCED. THE GENE WILL BE ISOLATED AND SEQUENCED. CRYSTALS WILL BE MADE WHICH WILL BE ANALYSED TO A RESOLUTION OF 2A USING SYNCHROTON RADIATION OR RAPID DATA ACQUISITION TECHNIQUES. COMPUTER MODEL OF THE ENZYME MOLECULE WILL BE CARRIED OUT AND WILL BE CONFIRMED BY NMR-STUDIES. PLANS FOR SITE DIRECTED MUTAGENESIS WILL BE WORKED OUT. MUTANT ENZYMES WILL BE PRODUCED AND ANALYSED. Dziedzina nauki engineering and technologymaterials engineeringcrystalsnatural sciencesearth and related environmental sciencesgeologymineralogycrystallographynatural scienceschemical sciencesinorganic chemistryalkaline earth metalsnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Program(-y) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Temat(-y) Data not available Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator NOVO INDUSTRI A/S Wkład UE Brak danych Adres NOVO ALLE 2880 Dania Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (1) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko University of York Zjednoczone Królestwo Wkład UE Brak danych Adres Heslington - YO1 5DD York Zobacz na mapie Koszt całkowity Brak danych
