Skip to main content
DeutschDE
European Commission logo print header
Inhalt archiviert am 2024-04-15

MICROBIAL PRODUCTION OF COMMERCIALLY IMPORTANT HYDROXYLATED COMPOUNDS FROM HALOAROMATICS

Ziel

DEVELOPMENT OF A NEW PROCEDURE BASED ON ARTHROBACTER ENZYMES FOR PRODUCTION OF THE FOLLOWING IMPORTANT HYDROXYLATED : 1,3,5, - TRIHYDROBENZENE, 4-HYDROXYPHENYLGLYCINE, TYROSINE, DOPA, 4-HYDROXYPHENLACETATE, M- AND P- HYDROXYBENZALDEHYDE, M- AND P-HYDROXYACETOPHENON AND 3,5-DIHYDROXYBENZOIC ACID.
At present there are no readily available preparative chemical reactions for direct, specific hydroxylation of aromatic compounds on an industrial scale. Consequently, the hydroxyl group has to be introduced by an expensive series of reactions. Certain microorganisms can, however, specifically dehalogenate and hydroxylate aromatic compounds. Thus, it may be possible to use microogranisms as biocatalysts.

The microbial transformation of a range of chlorobenzoates, chlorophenols, chlorobenzenes and chloroactophenones was studied. Of these substrates, chlorobenzoates yielded hydroxylated metabolites most readily. 4-chlorobenzoate (4-CBA) was investigated in greatest detail. Several bacteria were isolated which transformed 4-CBA into the target metabolite, 4-hydroxybenzoate. The growth of these organisms and the rate of 4-CBA transformation was enhanced by sequential subculture. Uptake studies suggest an active mechanism for transport of the substrate into the cell.
The enzyme responsible for 4-CBA dehalogenation and hydroxylation was extracted from the organism, partially purified and characterized. It was found to convert all p-halogenated benzoates into 4-hydroxybenzoate.
CONVERSION OF HALOGEN SUBSTITUTED AROMATIC COMPOUNDS INTO THE CORRESPONDING HYDROXYLATED COMPOUNDS BY USING THE NEWLY DISCOVERED ARTHROBACTER DEHALOGENATING/HYDROXYLATING ENZYME.

IN PARTICULAR :

1. THE GROWTH OF ARTHROBACTER ON DIFFERENT HALOGENATED AROMATIC COMPOUNDS WILL BE ASSESSED IN BATCH OR CONTINUOUS CULTURE
2. STUDY OF THE PRODUCTS PRODUCED BY NEW ANALYTICAL PROCEDURES (HIGH PRESSURE LIQUID CHROMATOGRAPHY AND GAZ CHROMATOGRAPHY).
3. ENRICHMENT OF APPROPRIATE STRAINS.
4. CHARACTERIZATION OF ENZYME ACTIVITY IN THE CELL FREE EXTRACT OF THE SELECTED MICROORGANISMS.
5. PURIFICATION AND OPTIMALIZATION OF THE SELECTED ENZYME ACTIVITY.

Wissenschaftliches Gebiet

Programm/Programme

Thema/Themen

Data not available

Aufforderung zur Vorschlagseinreichung

Data not available

Finanzierungsplan

CSC - Cost-sharing contracts

Koordinator

AGRICULTURAL UNIVERSITY WAGENINGEN
EU-Beitrag
Keine Daten
Adresse
DEPT. OF MICROBIOLOGY HESSELINK VAN SUCHTELENWEG 4
6703 CT WAGENINGEN
Niederlande

Auf der Karte ansehen
Gesamtkosten
Keine Daten

Beteiligte (1)

Diese Seite teilen

Herunterladen

Letzte Aktualisierung: 23 Januar 1994

Mein Booklet

Permalink: https://cordis.europa.eu/project/id/BAP*0043/de

European Union, 2024