Cel Light-sensitive channel proteins have attracted much attention as functional molecules, as they possess unique conformations and functions depending on their respective irradiation states. Embedding these proteins in heterogeneous cellular frameworks enabled to gain light-control of cellular and in particular neuronal behaviour. However to date, optogenetic solutions that address endogenous, intracellular biomolecules in a universal fashion remain elusive. The project will apply design and selection strategies aiming at nucleic acid molecules that can be controlled by irradiation with light and which we have developed in preliminary studies. This will now be taken significant steps forward by generating modular allosteric ribonucleic acid (RNA) assemblies that respond to light. These assemblies provide a generic solution and represent long-sought methods to complement the optogenetic toolbox. The aim of this project is the generation of allosteric molecules built from at least two RNA domains; one domain that binds to a soluble photoreceptor protein (PRP) in a light-dependent manner and a second RNA domain, whose protein inhibiting function in turn depends on the binding state of the PRP-recognizing part. We will construct light-responsive allosteric RNA assemblies that can be ubiquitously used in cells and in vivo, independent of specific model organisms, for optogenetic control and spatiotemporal analysis of endogenous, intracellular biomolecule function. The project is highly interdisciplinary and will open novel routes for biomolecule analysis in cells and in vivo. It has implications ranging from life sciences to optogenetics, and from combinatorial biochemistry to synthetic biology. As these new tools will be applicable by any scientist to analyse protein function at high spatiotemporal resolution, the project bears an enormous innovative potential. Dziedzina nauki natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNA Program(-y) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) ERC-CG-2013-LS9 - ERC Consolidator Grant - Applied Life Sciences and Non-Medical Biotechnology Zaproszenie do składania wniosków ERC-2013-CoG Zobacz inne projekty w ramach tego zaproszenia System finansowania ERC-CG - ERC Consolidator Grants Instytucja przyjmująca RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITAT BONN Wkład UE € 1 992 438,00 Adres REGINA PACIS WEG 3 53113 Bonn Niemcy Zobacz na mapie Region Nordrhein-Westfalen Köln Bonn, Kreisfreie Stadt Rodzaj działalności Higher or Secondary Education Establishments Kierownik naukowy Günter Mayer (Prof.) Kontakt administracyjny Daniela Hasenpusch (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych Beneficjenci (1) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITAT BONN Niemcy Wkład UE € 1 992 438,00 Adres REGINA PACIS WEG 3 53113 Bonn Zobacz na mapie Region Nordrhein-Westfalen Köln Bonn, Kreisfreie Stadt Rodzaj działalności Higher or Secondary Education Establishments Kierownik naukowy Günter Mayer (Prof.) Kontakt administracyjny Daniela Hasenpusch (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych