Objetivo The cellular environment is extremely complex and contains thousands of different bio-molecules. To determine how these bio-molecules assemble into a highly organized molecular system has remained a major challenge. Here, we aim to unravel how dozens of proteins assemble into a large molecular machinery that degrades mRNA in an efficient and regulated manner.On an atomic level, we will determine the static structures of the enzymes involved in the mRNA degradation pathways. In parallel, we will develop and exploit novel methods in NMR spectroscopy to correlate molecular motions with catalytic activity. Especially for the 450 kDa eukaryotic exosome complex, these studies will significantly push the limits of what is currently achievable. On a molecular level, we will reconstitute the mRNA degradation machinery from purified components in a stepwise manner. In this challenging approach, we will use NMR spectroscopy to follow how the enzymes engage in a network of interactions that regulate the mRNA degradation process. These studies will also provide us with the unique possibility to reproduce complex cellular behavior in a well defined and easy to manipulate in vitro system. On a microscopic level the mRNA degradation machinery assembles into cytoplasmic processing bodies. The role of these conserved cellular foci is a matter of debate and we aim to determine both the atomic details that result in this self-organization as well as the catalytic advantages that result from this clustering.Our study will provide a very detailed and accurate description of how essential and central molecular processes in mRNA degradation are regulated and modulated. The level of detail that we aim to achieve is currently not available for any cellular pathway of such complexity. In that regard, our projects also provide knowledge and methodology required to study additional and complex cellular functions. Ámbito científico natural sciencesphysical sciencesopticsspectroscopyabsorption spectroscopynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-CG-2013-LS1 - ERC Consolidator Grant - Molecular and Structural Biology and Biochemistry Convocatoria de propuestas ERC-2013-CoG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-CG - ERC Consolidator Grants Institución de acogida UNIVERSITAET REGENSBURG Aportación de la UE € 1 089 318,89 Dirección UNIVERSITATSSTRASSE 31 93053 Regensburg Alemania Ver en el mapa Región Bayern Oberpfalz Regensburg, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Matthias Köhler (Dr.) Investigador principal Remco Sprangers (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo UNIVERSITAET REGENSBURG Alemania Aportación de la UE € 1 089 318,89 Dirección UNIVERSITATSSTRASSE 31 93053 Regensburg Ver en el mapa Región Bayern Oberpfalz Regensburg, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Matthias Köhler (Dr.) Investigador principal Remco Sprangers (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV La participación finalizó Alemania Aportación de la UE € 877 481,11 Dirección HOFGARTENSTRASSE 8 80539 Munchen Ver en el mapa Región Bayern Oberbayern München, Kreisfreie Stadt Tipo de actividad Research Organisations Contacto administrativo Antje Lemper-Rupp (Mrs.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
