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Content archived on 2024-06-18

In Vivo Metabolite Modification in Hybrid Biological and Chemical Synthesis Systems

Objective

This proposal aims to integrate the fields of synthetic biology and synthetic organic chemistry. This project aims to achieve this through the application of biocompatible chemistry: non-enzymatic reactions capable of modifying small molecules in the presence of a living system. The idea of using microbial metabolites as reagents for non-enzymatic transformations in the presence of the producing organism will greatly expand the range of organic molecules that can be accessed using natural and engineered living systems.

We plan on applying a newly discovered engineered strain of E.coli that produces molecular hydrogen to important synthetic targets. We have demonstrated the use of this microbe in the presence of a palladium catalyst to hydrogenate a range of alkene- and alkyne-containing unnatural substrates. We propose to use this biocompatible hydrogenation reaction to the synthesis of industrially important advanced biofuels from biologically available starting materials. This would avoid the current need to extract the products of an engineered organism for further chemical manipulations, and would be the first example of the synthesis of these proposed molecules through purely biological means. Using bacterial olefin biosynthesis for the production of alkanes by interfacing single-/multi-system bacterial metabolism and a non-enzymatic, biocompatible hydrogenation reaction will represent an important advance in metabolic engineering and in biofuel manufacture.

We then plan to develop a batch- or flow-reactor set-up in order to investigate the viability of advanced biofuel synthesis using an engineered microbe/non-enzymatic chemistry synthesis systems for the bulk production of these important target molecules.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2013-IOF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IOF - International Outgoing Fellowships (IOF)

Coordinator

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
EU contribution
€ 282 109,20
Address
TRINITY LANE THE OLD SCHOOLS
CB2 1TN CAMBRIDGE
United Kingdom

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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